25-Hydroxylation of vitamin D3 in the human hepatoma cell lines Hep G2 and Hep 3B.

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25-Hydroxylation of vitamin D3 in the human hepatoma cell lines Hep G2 and Hep 3B.

Two human hepatoma cell lines, Hep G2 and Hep 3B, were screened for vitamin D3-25-hydroxylase enzyme activity by incubation with radioactive vitamin D3. A compound co-chromatographing with 25-OH-D3 was synthesized in both cell lines but its rate of synthesis was tenfold greater in Hep 3B than in Hep G2 cells. The identity of the compound was confirmed by comparing its chromatographic properties...

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The human hepatoma cell line Hep-G2 has been shown to express the major enzymes of intra- and extracellular cholesterol metabolism. These include lecithin:cholesterol acyltransferase, acyl coenzyme A:cholesterol acyltransferase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and cholesterol-7 alpha-hydroxylase. Regulatory mechanisms that have been described in other hepatic systems also appea...

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Receptor-mediated endocytosis of tissue-type plasminogen activator (t-PA) was characterized with the human hepatoma cell line Hep G2. At 4 degrees C binding of 125I-t-PA to Hep G2 cells is rapid, specific, saturable, and reflective of a homogeneous population of 76,000 high-affinity surface sites per cell (Kd = 3.7 nM). The kinetics of 125I-t-PA binding to its receptor are characterized by rate...

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Evaluation of the single cell gel electrophoresis assay with human hepatoma (Hep G2) cells.

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Single-cell gel electrophoresis assays with human-derived hepatoma (Hep G2) cells.

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1988

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)38405-4